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KMID : 0981820080280010001
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Korean Journal of Laboratory Medicine
2008 Volume.28 No. 1 p.1 ~ p.7
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Distribution of Antigenic Aberration in the Bone Marrow of Acute Leukemia in Complete Remission
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Shin So-Young
Kim Myung-Shin
Lim Ji-Hyang
Kim Yong-Goo
Han Kyung-Ja
Kahng Ji-Min
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Abstract
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Background : The aberrant, leukemia-associated antigen expression patterns allow us to discriminate leukemic blasts from normal precursor cells. Our major goal was to determine a guideline for the detection of minimal residual disease using CD20+/CD34+ and myeloid Ag+/CD19+ combination in the bone marrow of acute leukemia in complete remission (CR) after chemotherapy.
Methods : Bone marrow samples from 117 patients with acute leukemia in complete remission after chemotherapy and from 22 healthy controls were immunophenotyped by triple staining and measured by flow cytometry .
Results : The CD20+/CD34+ cells in the large lymphocyte gate (R1) ranged from 0% to 3.24% (0.8±0.82%, P=0.000) in CD20+/CD34+ B-lineage ALL CR (N=31), from 0.03% to 4.2% (0.7±0.83 %, P=0.000) in CD20-/CD34- B-lineage ALL CR (N=66), from 0.1% to 0.96% (0.45±0.32%, P=0.016) in T-ALL CR (N=10), and from 0.02% to 0.48% (0.18±0.15%, P=0.776) in AML CR (N=10). The CD13,33+/CD19+ cells in R1 gate ranged from 0% to 2.69% (0.37±0.48%, P<0.001) in CD13,33+/ CD19+ B-lineage ALL CR (N=31), from 0% to 1.8% (0.31±0.28%, P<0.001) in CD13,33-/CD19+ B-lineage ALL CR (N=65), from 0.02% to 0.64% (0.29±0.22%, P=0.071) in T-ALL CR (N=9), and from 0% to 0.17% (0.07±0.09%, P=0.341) in AML CR (N=3).
Conclusions : Using an immunophenotypic method for the detection of early relapse or minimal residual disease of B-lineage ALL bone marrow in CR after chemotherapy, different cutoff values should be applied according to antigen combination and gating. When the proportion of aberrant antigen combination was less than 5% in large lymphocyte gate, the results should be interpreted with caution. (Korean J Lab Med 2008;28:1-7)
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KEYWORD
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Aberrant antigen, CD20+/CD34+, Myeloid Ag+/CD19+
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